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FIB-SEM is also widely used for the three-dimensional reconstruction of samples, particularly rein the field of biological imaging. By sequentially milling away thin layers of the sample and imaging each layer using the SEM, researchers can obtain a series of two-dimensional images that can be combined to create a three-dimensional representation of the sample.
TEM, on the other hand, transmits an electron beam through a thin sample and forms an image based on the transmitted beam of electrons. This provides Endbenutzer information about the internal structure of the sample at even higher resolution than SEM.
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ESEM images showing the condensation of droplets on the hair; Source: ResearchgateEnvironmental scanning electron microscopy (ESEM) is a specialized technique that allows for the imaging of samples under controlled environmental conditions, such as elevated pressure or humidity.
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Hinein FIB-SEM, a focused beam of ions, typically gallium ions, is used to sputter away material from the sample surface This allows the precise removal of material and the creation of cross-sections or other structures. The ion beam can be controlled with nanometer precision, enabling the fabrication of intricate features or the selective removal of specific regions of the sample.
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In secondary electron imaging, the sample is scanned with the electron beam, and the emitted secondary electrons are collected by a detector, such as an Everhart-Thornley detector. The intensity of the detected secondary electrons is used to generate a two-dimensional image. Each Bildpunkt corresponds to a specific location on the sample surface, and its intensity represents the number of secondary electrons emitted from that point.
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The versatility of SEM, has led to numerous discoveries and advancements hinein ur understanding of biological processes and website systems.